Journal: Heliyon

Article Title: miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats

doi: 10.1016/j.heliyon.2024.e26572

Figure Lengend Snippet: Primers used for RT-qPCR analysis.

Article Snippet: The protein samples were then transferred to a PVDF membrane (0.45-μm pore size) at a constant pressure of 100 V for 10 min, followed by blocked overnight in Tris-buffered saline with 0.1% Tween-20 (TBST), which contained 5% bovine serum albumin, and then probed overnight at 4 °C with primary antibodies against PPARβ (1:2000; GTX113250) and FAS (1:1000; GTX13550) (both from GeneTex, Irvine, CA, USA) and ACC2 (1:500; A03668-2) and CPT1b (1:500; PB9491) (both from Boster Bio, Pleasanton, CA, USA).

Techniques: Amplification

Journal: Heliyon

Article Title: miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats

doi: 10.1016/j.heliyon.2024.e26572

Figure Lengend Snippet: Expression of the lipid metabolism regulator PPARβ and downstream effectors (CPT1b, FAS, and ACC2). mRNA levels in obese rats under hypoxic conditions, with hypoxic training, and with regulated miR-122 expression were determined using qRT-PCR. OE, obese rats with miR-122 overexpression and hypoxic training; IE, obese rats with miR-122 depletion and hypoxic training; CE, obese rats with hypoxic training only; H, obese sedentary rats without regulation of miR-122 expression. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 vs rats in group H; # p < 0.05, ## p < 0.01 vs rats in group CE; & p < 0.05, && p < 0.01 vs rats in group OE (1-way analysis of variance).

Article Snippet: The protein samples were then transferred to a PVDF membrane (0.45-μm pore size) at a constant pressure of 100 V for 10 min, followed by blocked overnight in Tris-buffered saline with 0.1% Tween-20 (TBST), which contained 5% bovine serum albumin, and then probed overnight at 4 °C with primary antibodies against PPARβ (1:2000; GTX113250) and FAS (1:1000; GTX13550) (both from GeneTex, Irvine, CA, USA) and ACC2 (1:500; A03668-2) and CPT1b (1:500; PB9491) (both from Boster Bio, Pleasanton, CA, USA).

Techniques: Expressing, Quantitative RT-PCR, Over Expression

Journal: Heliyon

Article Title: miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats

doi: 10.1016/j.heliyon.2024.e26572

Figure Lengend Snippet: Expression of the lipid metabolism regulator PPARβ and downstream effectors (CPT1b, FAS, and ACC2). Protein levels in obese rats under hypoxic conditions, with hypoxic training, and with regulated miR-122 expression were determined using Western blot analysis. OE, obese rats with miR-122 overexpression and hypoxic training; IE, obese rats with miR-122 depletion and hypoxic training; CE, obese rats with hypoxic training only; H, obese sedentary rats without regulation of miR-122 expression. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 vs rats in group H; # p < 0.05, ## p < 0.01 vs rats in group CE; & p < 0.05, && p < 0.01 vs rats in group OE (1-way analysis of variance).

Article Snippet: The protein samples were then transferred to a PVDF membrane (0.45-μm pore size) at a constant pressure of 100 V for 10 min, followed by blocked overnight in Tris-buffered saline with 0.1% Tween-20 (TBST), which contained 5% bovine serum albumin, and then probed overnight at 4 °C with primary antibodies against PPARβ (1:2000; GTX113250) and FAS (1:1000; GTX13550) (both from GeneTex, Irvine, CA, USA) and ACC2 (1:500; A03668-2) and CPT1b (1:500; PB9491) (both from Boster Bio, Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Over Expression

Journal: Annals of palliative medicine

Article Title: Down-regulated expression of transient receptor potential ankyrin 1 in lichen simplex chronicus.

doi: 10.21037/apm-20-1712

Figure Lengend Snippet: Figure 1 Protein expression of TRPA1 in the LSC group and control group. (A) Western blot results for TRPA1 expression in skin tissues

Article Snippet: The proteins in a sample were separated using sodium dodecyl sulfate-polyacrylamide gel (Beyotime Biotech, Shanghai, China) electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Beyotime Biotech, Shanghai, China) at a constant voltage of 80 V. After being blocked with a rapid blocking buffer for 10–15 minutes, and the membrane was incubated at 4 °C in an ice room overnight with primary antibodies against TRPA1 (1:1,000; ACC-037, Alomeno Labs, Israel) and β-actin (1:500; Wuhan Boster Biological Technology, China).

Techniques: Expressing, Control, Western Blot

Journal: Annals of palliative medicine

Article Title: Down-regulated expression of transient receptor potential ankyrin 1 in lichen simplex chronicus.

doi: 10.21037/apm-20-1712

Figure Lengend Snippet: Figure 2 Expression of TRPA1 in the LSC group and control group by Immunohistochemical analysis. (A) The expression of TRPA1 in skin tissue of

Article Snippet: The proteins in a sample were separated using sodium dodecyl sulfate-polyacrylamide gel (Beyotime Biotech, Shanghai, China) electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Beyotime Biotech, Shanghai, China) at a constant voltage of 80 V. After being blocked with a rapid blocking buffer for 10–15 minutes, and the membrane was incubated at 4 °C in an ice room overnight with primary antibodies against TRPA1 (1:1,000; ACC-037, Alomeno Labs, Israel) and β-actin (1:500; Wuhan Boster Biological Technology, China).

Techniques: Expressing, Control, Immunohistochemical staining

Journal: Annals of palliative medicine

Article Title: Down-regulated expression of transient receptor potential ankyrin 1 in lichen simplex chronicus.

doi: 10.21037/apm-20-1712

Figure Lengend Snippet: Figure 3 TRPA1 and inflammatory mediators in the skin tissue specimens mRNA expression in the LSC group and control group. (A)

Article Snippet: The proteins in a sample were separated using sodium dodecyl sulfate-polyacrylamide gel (Beyotime Biotech, Shanghai, China) electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Beyotime Biotech, Shanghai, China) at a constant voltage of 80 V. After being blocked with a rapid blocking buffer for 10–15 minutes, and the membrane was incubated at 4 °C in an ice room overnight with primary antibodies against TRPA1 (1:1,000; ACC-037, Alomeno Labs, Israel) and β-actin (1:500; Wuhan Boster Biological Technology, China).

Techniques: Expressing, Control